精原干细胞研究组！
APP宣发
韩春生 研究员
生殖细胞形成和发育的基因调控
欢迎您加入精原干细胞研究组！
– 工作人员：林羲雯（生物信息学分析师,实验室主管）
– 研究生：谢丹，刘元，宋雪，贺伟，李亚欢，赵少康，闫琳，刘晓薇，胡帅涛，薛亚林，孙静, MD Alim, , Khan
动物的精子发生是指性腺中一小群叫做精原干细胞（SSCs）的细胞经过复杂的增殖、分化、减数分裂最终形成精子的过程。在胚胎发育早期，少数上胚层细胞首先形成原始生殖细胞，这些早期的生殖细胞经过增殖、迁徙和分化最终形成了SSCs。SSCs不仅能够经过分化变为能进行减数分裂的精母细胞，而且还能够重编程形成多能性干细胞。因此，SSCs是研究干细胞自我更新、分化、去分化、以及减数分裂的优秀细胞模型。精子发生的缺陷会导致男性不育和出生缺陷，而对其进行干预则可能发展出避孕、不育治疗、出生缺陷矫正、动物繁育、再生医学等方面的新技术。我们目前正在利用分子生物学、小鼠基因敲除、高通量组学和生物信息学等技术来研究SSCs增殖、分化、重编程以及利用多能性干细胞获得生殖细胞的分子机理。
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研究内容和目标:
1. 精原干细胞（SSCs）增殖、分化（减数分裂）以及去分化（重编程）研究。我们已经建立了精原干细胞的体外培养扩增以及多能性干细胞诱导形成生殖细胞的平台，为发展基于人工诱导生殖细胞的不育治疗技术奠定了基础。未来将面向男性不育治疗的应用目标，快速推进在体外获得“人造”精子的工作。
2. 精子发生的表观遗传调控机理。我们已经利用分离纯化的多种生精细胞结合组学技术，在DNA、RNA、蛋白质三个水平绘制哺乳动物精子发生基因调控的全局视图，利用基因敲除和分子生物学技术重点研究关键转录因子在精子发生中的功能，为男性不育的诊断提供了候选基因。未来我们将继续利用组学技术研究在生殖细胞形成和发育过程中基因表观遗传修饰的变化以及RNA和蛋白质的表达变化，发现对分化起关键作用的基因，并针对其作用机理设计出更加有效的体外分化诱导方案，为临床转化奠定基础。
代表性发表论文:
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